cholesterol liquid reagents kits  (Pointe Scientific)

Journal: bioRxiv

Article Title: Dietary depletion of glutamine is atheroprotective

doi: 10.64898/2026.03.06.710174

Figure Lengend Snippet: ( A ) Experimental design. Apoe -/- male mice were fed WD and received drinking water with no additives (Ctrl) or water supplemented with glutamine (Gln), or alpha-ketoglutarate (AKG) for 8 weeks. ( B ) Oil red O analysis of plaque burden in the aorta with ( C ) representative images of aortas from male mice. Scale bar: 2 mm. ( D ) Plaque area at 7 locations across the aortic root from the first appearance of the aortic valves in (top) male and (bottom) female mice. ( E ) Representative images of aortic root plaques from male and female mice in control (Ctrl) and glutamine (Gln) group. Scale bar: 200 μm. Black dashed line marks representative plaques boundaries. ( F ) Total plaque volume in the aortic root, calculated as the area under the curve from (D). ( G ) Sirius red analysis of plaque collagen content. ( H ) Necrotic core size analysis at the point of peak stenosis. ( I ) Plasma cholesterol. ( J ) Glutamine plasma concentration normalized to sex matched controls. (B, F, G, I, J) Data analyzed using one-way ANOVA with Dunnett’s correction or (H) Kruskal Wallis with Dunn’s correction for post-hoc analysis with N ≥ 13. Error bars represent mean ± SEM or (H) median ± IQR. *** P < 0.001; ** P = 0.001 to 0.01; * P = 0.01 to 0.05; ns, not significant.

Article Snippet: Plasma cholesterol was analyzed, using Infinity Cholesterol Liquid Stable Reagent kit (Thermo Scientific; cat#: TR13421) and Data-Trol A, Abnormal Control Serum (Thermo Scientific; cat#: TR41001), as an internal standard, according to the manufacturer’s instructions.

Techniques: Control, Clinical Proteomics, Concentration Assay

Journal: bioRxiv

Article Title: Targeting Microbial Bile Salt Hydrolase Reprograms Bile Acid Metabolism and Ameliorates Metabolic Dysfunction–Associated Steatohepatitis in Mice

doi: 10.64898/2026.05.12.724693

Figure Lengend Snippet: a , Liver weight. b , Liver-to-body weight ratio. c , Total hepatic triglyceride content. d , Total hepatic cholesterol content. e , Quantification of Oil Red O–positive area. f , Plasma alanine aminotransferase (ALT) levels. g , Plasma lipopolysaccharide (LPS) levels. h , Hepatic hydroxyproline content. i , Quantification of Sirius Red–positive area. j , Representative histological images of liver sections from chow diet (CD), western diet (WD), early-intervention low-dose (EI-Low), early-intervention high-dose (EI-High), late-intervention low-dose (LI-Low), and late-intervention high-dose (LI-High) groups. Top row: H&E staining; middle row: Oil Red O staining; bottom row: Sirius Red staining. Scale bars, 50 μm. Data are shown as mean ± SD. Each symbol represents one mouse. Statistical significance was determined by one-way ANOVA with Bonferroni’s multiple-comparisons test. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Article Snippet: Hepatic triglyceride and cholesterol concentrations were quantified using Triglyceride Liquid Reagents and Cholesterol Liquid Reagents kits, respectively (Pointe Scientific).

Techniques: Clinical Proteomics, Western Blot, Staining

Journal: American journal of physiology. Gastrointestinal and liver physiology

Article Title: Retatrutide Improves Steatohepatitis in an Accelerated Mouse Model of Diet-Induced Steatohepatitis with a Fructose Binge

doi: 10.1152/ajpgi.00164.2025

Figure Lengend Snippet: For 31 days, female C57BL/6N mice were fed either chow diet and regular drinking water (Control), western diet and regular drinking water (Western Diet), western diet and drinking water with added fructose and sucrose (Western Diet + F/S), or western diet and drinking water with added fructose and sucrose plus a final fructose binge (10 mg/g body weight) 6 hours before sacrifice (Western Diet + F/S + Binge) (n=12 per group). (A) Absolute liver weight. (B) Liver weight-to-body weight ratio. (C) Plasma alanine aminotransferase content. (D) Hepatic triglyceride content. (E) Hepatic cholesterol content. (F-H) Hepatic gene expression of (F) Cxcl-1 , (G) Saa-1 , and (H) Saa-2 . (I) Hepatic collagen content. (J-K) Representative liver sections after (J) hematoxylin and eosin staining (bar size = 100 μ m) and (K) after Oil Red O staining (bar size = 100 μ m). Results are expressed as mean ± s.e.m. P values are determined by 1-way ANOVA, corrected for multiple comparisons using statistical hypothesis testing by Holm-Šídák (A-B), or Kruskal-Wallis with Dunn’s post hoc test (C-I). * P <0.05. ALT, alanine aminotransferase; Cxcl-1 , chemokine ligand-1; Saa-1 , serum amyloid A1; Saa- 2, serum amyloid A2.

Article Snippet: Hepatic cholesterol levels were measured using the Cholesterol Liquid Reagents Kit (Pointe Scientific, Canton, MI).

Techniques: Control, Western Blot, Clinical Proteomics, Gene Expression, Staining

Journal: American journal of physiology. Gastrointestinal and liver physiology

Article Title: Retatrutide Improves Steatohepatitis in an Accelerated Mouse Model of Diet-Induced Steatohepatitis with a Fructose Binge

doi: 10.1152/ajpgi.00164.2025

Figure Lengend Snippet: Female C57BL/6N mice were fed over 31 days either with chow diet (n=5 per group) and regular drinking water or western diet with added fructose and sucrose in the drinking water and a final fructose binge 6 hours prior to sacrifice (n=15 per group) with or without retatrutide treatment (30 nmol/kg body weight) on days 15, 18, 21, 24, 26, 28, and 30. (A) Absolute liver weight. (B) Liver weight-to-body weight ratio. (C) Plasma alanine aminotransferase content. (D) Hepatic triglyceride content. (E) Hepatic cholesterol content. (F-I) Hepatic gene expression of (F) Cxcl-1 , (G) Saa-1 , and (H) Saa-2 . (I-J) Representative liver sections after (I) hematoxylin and eosin staining (bar size = 100 μ m) and (J) after Oil Red O staining (bar size = 100 μ m). Results are expressed as mean ± s.e.m. P values are determined by 1-way ANOVA, corrected for multiple comparisons using statistical hypothesis testing by Holm-Šídák (A-E), or Kruskal-Wallis with Dunn’s post hoc test (F-H). * P <0.05. ALT, alanine aminotransferase; Cxcl-1 , chemokine ligand-1; Saa-1 , serum amyloid A1; Saa- 2, serum amyloid A2.

Article Snippet: Hepatic cholesterol levels were measured using the Cholesterol Liquid Reagents Kit (Pointe Scientific, Canton, MI).

Techniques: Western Blot, Clinical Proteomics, Gene Expression, Staining

Journal: International Journal of Neonatal Screening

Article Title: Analytical Validation of Familial Hypercholesterolemia Biomarkers in Dried Blood Spots

doi: 10.3390/ijns8010014

Figure Lengend Snippet: Assay precision and recovery of total cholesterol, low-density lipoprotein-cholesterol, and apolipoprotein B from dried blood spot controls. CV, coefficient of variation; N, total number of evaluations.

Article Snippet: Methanol (120 μL) was added to each well of the TC and LDL-C plates for extraction of biomarkers and the plate was incubated with shaking at 37 °C for 30 min. Step-wise enzymatic assays to measure TC were performed, with modifications, using the cholesterol liquid reagent kit from Pointe Scientific, Canton, MI, USA (cat# C7510-500).

Techniques: Clinical Proteomics, Concentration Assay, Standard Deviation

Journal: International Journal of Neonatal Screening

Article Title: Analytical Validation of Familial Hypercholesterolemia Biomarkers in Dried Blood Spots

doi: 10.3390/ijns8010014

Figure Lengend Snippet: Limits of detection and quantification, and linearity of total cholesterol, low-density lipoprotein-cholesterol, and apolipoprotein B in dried blood spots.

Article Snippet: Methanol (120 μL) was added to each well of the TC and LDL-C plates for extraction of biomarkers and the plate was incubated with shaking at 37 °C for 30 min. Step-wise enzymatic assays to measure TC were performed, with modifications, using the cholesterol liquid reagent kit from Pointe Scientific, Canton, MI, USA (cat# C7510-500).

Techniques:

Journal: International Journal of Neonatal Screening

Article Title: Analytical Validation of Familial Hypercholesterolemia Biomarkers in Dried Blood Spots

doi: 10.3390/ijns8010014

Figure Lengend Snippet: Comparison of Serum to DBS Assays. The Bland–Altman plots show the variation in the serum as compared to the DBS concentration for specimens collected concurrently in 48 research subjects. ( A ) Total cholesterol; ( B ) low-density lipoprotein-cholesterol; ( C ) apolipoprotein B. Red line, bias; dashed Line, 95% limits of agreement.

Article Snippet: Methanol (120 μL) was added to each well of the TC and LDL-C plates for extraction of biomarkers and the plate was incubated with shaking at 37 °C for 30 min. Step-wise enzymatic assays to measure TC were performed, with modifications, using the cholesterol liquid reagent kit from Pointe Scientific, Canton, MI, USA (cat# C7510-500).

Techniques: Comparison, Concentration Assay

Journal: International Journal of Neonatal Screening

Article Title: Analytical Validation of Familial Hypercholesterolemia Biomarkers in Dried Blood Spots

doi: 10.3390/ijns8010014

Figure Lengend Snippet: Evaluation of presumptively unaffected population. Multiples of the median for total cholesterol (TC), low-density lipoprotein (LDL-C), and apolipoprotein B (ApoB) in 820 newborn specimens collected within 24–72 h after birth.

Article Snippet: Methanol (120 μL) was added to each well of the TC and LDL-C plates for extraction of biomarkers and the plate was incubated with shaking at 37 °C for 30 min. Step-wise enzymatic assays to measure TC were performed, with modifications, using the cholesterol liquid reagent kit from Pointe Scientific, Canton, MI, USA (cat# C7510-500).

Techniques: